| Summary: | 碩士 === 輔仁大學 === 生命科學系碩士班 === 102 === Aim：Laser refractive surgery is commonly used to treat myopia, hyperopia, astigmatism. The present study is to explore the postoperative corneal wound healing process: the possible role of Moxifloxacin (MOX) in secretion, migration and proliferation.
Methods：TGF-β1 modulates wound environment, cultured human corneal fibroblasts (HCF) and uses dot blotting and Western blotting to analyze the expression of interleukin-12, and possible regulatory mechanisms for MOX. Observe the migration rate under culturing HCF in MOX treatment. Final analysis functional of endogenous FBLN1 in HCF, cultured HCF growth curve in MOX treatment, and the factors (JNK, p53, p21, Bcl-2, Bcl-xL and survivin) that may affect proliferation.
Results and discussion：The medium was collected after dot blotting analysis of experimental results of the second day will be apparent induction by TGF-β1, in particular to the day 3 be induced a large number, which is about the first day of 3.7 times. The TGF-β1-induced phenomena will be suppressed down by MOX. HCF with increasing concentrations of MOX and slowed the rate of migration. MOX inhibits the expression of endogenous FBLN1, in the day 3 reduced from 8 times to 3.5 times, HCF the growth rate is also reduced by 3 times to 1.7 times. MOX suppressed the expression of endogenous FBLN1, affect the functionality of endogenous FBLN1, the cell adhesion force variation, to inhibit the proliferative effect. MOX activation of JNK, increased the expression of p-JNK; increased Bcl-2, Bcl-xL and survivin expression levels, which may increase the anti-apoptotic ability; although also increased the expression of p53 and p21, but no obvious apoptosis phenomenon, but the HCF growth rate slowed.
Conclusion：MOX may play a role in its antibiotic, inhibits HCF secretion, the migration and proliferation. The results for corneal wound healing potential benefits: significantly reduces the risk of postoperative infection, but also reduces the possibility of corneal cell aging, making corneal wound to heal slowly.
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