| Summary: | 碩士 === 逢甲大學 === 材料科學與工程學系 === 101 === Peptide is composed of amino acids, and can attract Ca2+ and HPO32- from the SBF (Simulated body fluid) through the positive and negative electric charges in the peptide. Using it in biochemical modification can accelerate the formation of an apatite layer. Our Experiment employed the Aspartic-Serine- Serine Sequence repeated three times (3DSS) to promote the apatite layer on Ti-6Al-4V.
The XRD pattern of a calcium phosphate prepared using 3DSS 10, 30, 50, 100 and 400 M did not show any obvious peaks at (002) and (211), indicating that no periodicity was present in the calcium phosphate coating, and the layer induced by 3DSS 200 M had a wide diffraction peak at the (002) and (211) planes, means the calcium phosphate frameworks has low crystalline, and the FTIR spectrum showed an obvious peak at 874 cm-1 due to the ionized carboxyl groups in the calcium phosphate coating.
According to the contact angle test, the apatite layer induced by 3DSS 200 M treatment has the smallest contact angle. However, it shows the most improved corrosion-resistant ability.
Human osteoblast-like MG63 cells were cultured on a calcium phosphate layer prepared using 3DSS 30, 200 M for 1 hour. The result shows that, after treatment with 3DSS 30 M, the MG-63 cells on the apatite-coated layer were flatter than that of Ti-6Al-4V, and treatment with 3DSS 200 M shows a lot of filopodia extending from the MG-63 cells, which were found to tightly adhere to the coating surface, suggesting good cell viability.
|
|---|
