| Summary: | 碩士 === 大葉大學 === 環境工程學系碩士班 === 101 === In recent years, the increasing concentration of carbon dioxide in the atmosphere has been considered one of the main cause for global warming. People began to study ways to reduce carbon dioxide emission. Cultivation of microalgae is an potential method for fixing carbon dioxide. This study intends to investigate the effects of sodium bicarbonate concentration, temperature and light intensity on the growth of Nannochloropsis sp.. For batch cultures of Nannochloropsis sp., the growth rate and the specific growth rate were used to identify the suitable for growth conditions by one-factor method. The major events are as follows:
(1) Cultivations were conducted with sodium bicarbonate concentration in the range of 1 to 30 g / L. The growth rate of Nannochloropsis sp. increased with the increasing concentration of sodium bicarbonate up to 15 g/L. The growth inhibition was observed as the concentration over 20 g/L. The optimal concentration of sodium bicarbonate was 15 g/L.
(2) Tests were conducted with temperature in the range of of 20 to 40℃. The growth rate of Nannochloropsis sp. increased with the increasing temperature up to 30℃. The growth inhibition was also observed as the temperature greater than 35℃. The optimal temperature was 30℃.
(3) For Cultivations of Nannochloropsis sp., light intensity range was from 2000 to 20000 Lux. The growth rate of Nannochloropsis sp. increased with the increasing light intensity up to 15000 Lux. The growth inhibition was also observed as the light intensity greater than 15000 Lux.
(4) A high biomass concentration culture was conducted at the optimum conditions. The biomass concentration was up to 1.8 g/L with the initial biomass concentration of 1.4 g/L .
Keyword: Nannochloropsis sp., sodium bicarbonate, light intensity
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