Effects of Strains and Media on Activities of Ligninolytic Enzymes and Decolorization Treatment for Dye Waste Water by Trametes versicolor Submerged Fermentation

• Main Authors: Zi-Yao, Zhou, 周子耀
• Other Authors: Fang-Yi, Lin
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/35636967462303004671

碩士 === 大葉大學 === 生物產業科技學系 === 101 === Trametes versicolor is a medicinal fungus, also known as white rot fungi. T. versicolor contains multiple extracellular enzyme systems that degrade organic matter in the environment, especially lignin., The biodegradable matters were then used for mycelial growth, by secreting laccase (laccase; EC1.10.3.2), manganese peroxidase (EC 1.11.1), xylanase (EC 3.2.1.8) during the fermentation processes. Those enzymes showed effects on lignin degradation, however, different T. versicolor strains isolated from Taiwan showed different lignin degradation activities. In the fermentation processes, the compositions of fungal culture medium is very important, the agricultural nutrient sources in the medium are more complex than chemical medium. Many pevious reserches replaced half of culture medium with agriculture compartments to increase the fungal metabolites such as fungi polysaccharides. In this thesis, Taiwan strains T. versicolor BCRC35644, BCRC35759, BCRC35682, BCRC 35683 and LH1 were tested for multiple enzyme activities using differet fermentation mediums. As for the laccase activity in liquid fermentation comparisons, the results of chemical medium were 23U/mL, 35U/mL, 61U/mL, 76U/mL, 37 U / mL, respectively. As for the laccase activities in agricultural medium, were 81 U / mL, 69 U / mL, 282 U / mL, 188U/mL, 342 U / mL, respectively. Phenol blue agar was then used to exam the manganese peroxidase avtivities of LH1 and BCRC35682. The results showed that T. versicolor LH1 has a better manganese peroxidase activity. To further explore the manganese peroxidase activity of T. versicolor LH1 in fermentation processes, T. versicolor LH1 was subjested used in a fermentor. The result showed that in the highest manganese peroxidase activity was obtained in the first six days with the activity of 36.654U/mL, highest xylanase activity was also obtained during the first six days with the activity of 2.5U/ml. Bleaching experiments were carried out by adding clothes dyes to the diluted culture medium to explore the free agent mycelium decolorization ability, the result showed that free mycelia decolorization capacity can up to 95%. Finally, T. versicolor LH was used on different stages of processed dying waste water to test for its decolorization abilities, the results showd that T. versicolor LH had a good decolorzation ability. The experimental results show that agriculture medium can increased laccase activity by 2 to 10 times higher than chemical medium. The highest activity of manganese peroxidase, xylanase were also obtained during the first six days of culture. T. versicolor LH1 also showed good performance on bleaching activity. This thesis provides valuable results for industrial applications, in the future, with the improvement of the enzyme purity, laccase from T. versicolor LH1 in wastewater treatment will be very valuable and significance.