| Summary: | 碩士 === 朝陽科技大學 === 應用化學系生化科技碩士班 === 101 === Antrodia camphorata (AC) is a unique mushroom of Taiwan. It parasite on the protected endemic species Cinnamomum kanehirae (Bull camphor tree).The extracted triterpenoids from A. camphorata express excellent bioactivities, like anti-cancer, anti-inflammatory, lowering-blood pressure, lowering-blood lipids and activate nerve cells and other effects. It was widely used in functional (health) foods and medicine development in Asia.
Currently, the products of A. camphorata on market come from different sources. Based on liquid culture or solid-state cultured condition, the triterpenoid components are also different. Usually, triterpenoids was analyzed by HPLC, LC-MS/MS was also used to do pharmacokinetic study of triterpenoids in rat blood. No study reports the application of MALDI-TOF MS on analysis of A. camphorata. In this study, a fast, sensitive and enviromental friendly analysis method by MALDI-TOF MS will be applied to determine eight triterpenoid componds of A. camphorata. A octadecyltrichlorosilane (OTS) modified hydrophobic aluminum foil surface was used on target plate to increase the analyte signal intensity. The identified analytes include methyl antcinate B (AC 1), antcin B (AC 5), antcin H (AC 7), antcin A (AC 9), antcin C (AC 10), methyl antcinate A (AC 11), antcin K (AC 12), and methyl antcinate H (AC 13). The results, showing the linear range and r2 value are 2 ppm~20 ppm with r2 = 0.954 ~ 0.996, respective. This method will be able to fast distinguish the different sources of A. camphorata products on market.
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