Synthesis and characterization of carboxylic fluorescence dyes and mesoporous silica materials with dye

• Main Authors: Shan-Mei Tseng, 曾善美
• Other Authors: Chi-Feng Cheng
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/41788223867786631707

碩士 === 中原大學 === 奈米科技碩士學位學程 === 101 === Abstract Commercially available 5(6)-Carboxyfluorescein with NCS-functional groups is very expensive and has a fixed green fluorescence. Therefore, four inexpensive fluorescent agents containing additional carboxylic acid groups were prepared via Friedel-Crafts reaction. The first dye is F, which emits green fluorescence and commonly used in biological labeling. B is the second dye and emits the orange fluorescence. The third 123 emits yellow-green fluorescence and last G emits yellow fluorescence. Nano-sized nanoporous materials tethering carboxylic indicators were prepared via direct-synthesis and co-condensation. Indicators with carboxylic group were synthesized on nanoporous materials via Friedel-Crafts reaction. These nanoporous materials with indicators can be used for trace of biological cells.Two kinds of nanosized nanoporous material were synthesized. One was MSN (mesoporous silica nanoparticle) with porosity of 2.6 nm and particle size of 70 nm. Another was SBA-15NPs (SBA-15 nanoparticles) having 10 nm porous and 400 nm particle size. Indicators on MSN and SBA-15NPs were bonded after synthesis. Rh123 was bonded on the surface of nanoporous nanoparticles via in-situ Friedel-Crafts reaction and emits strong yellow-green fluorescence. The characteristic fluorescence intensity variation with various pH values in solution was monitored by photography and fluorescence spectroscopy. MSNs with indicators were effectively endocytosed into cells as cell markers. Synthesized nanoporous materials with indicators via Friedel-Crafts reaction are inexpensive, simple and rare as compared to commercially available dyes. Synthesis of different indicators can be achieved by simply adding different reactants. In addition, Silica as the component of the synthesized nanomaterial has high biocompatibility and stability. Thus, mesoporous nanoparticles with indicators can be applied not only as a cell maker but also a medicine carrier.