| Summary: | 碩士 === 中原大學 === 生物科技研究所 === 101 === The application of nanofibers in tissue engineering offers an option and hope of cellular therapy, especially in the central nervous system. A novel nano substrate poly-(o-methoxyaniline)(POMA) were seeded with neural stem cells and found to have more differential efficiency compared with PDL. The epigenetic mechanism of neural stem cells differentiation on POMA had been analyzed by PSSH before. Hence, to understand its properties, differentially expressed miRNAs targets of neural stem cells on POMA, PDL-coated glass were identified by microRNA microarray. We clustered miRNA target genes via Ingenuity Pathway Analysis (IPA) based on their functions and roles in neural cell differentiation. Sixteen differentially expressed genes were selected with significant role in neural stem cells differentiation in both POMA and PDL: eight (CYTH2, DOCK1, FGFR1, FNBP1, MSN, PACSIN1, SEPT11, RIMS4) in neuritogenesis, four (ERBB2, RB1, CPEB1, IGF-1) in proliferation and four (ERBB2, NCAM1, RIMS4, IGF-1) in differentiation. However, three genes had opposite regulation direction in POMA and PDL, which are L1CAM, NFASC and NTRK2. These predicted genes need further confirmations. In conclusion, POMA fibers could be a candidate as a scaffold for NSC differentiation in miRNAs level.
|
|---|
