| Summary: | 碩士 === 中臺科技大學 === 醫學工程暨材料研究所 === 101 === Yttria-Tetragonal Zirconia Polycrystal(Y-TZP) is a preference material used as biomaterials due to its good mechanical properties. In order to improve the biocompatibility of zirconia, RGD-peptide derived from extracellular matrix proteins was employed to modify the surface of YTZP to promote cell adhesion.
In this study, the surfaces of YTZP were modified using hydrothermal method at different period. The topographies of modified YTZP were analyzed by contact angle, XRD, FTIR, AFM and FE-SEM, as well as the mechanical properties were evaluated vickers hardness, fracture toughness and three point bending. The RGD-peptide was immobilized on the surface of YTZP by chemical treatment and hydrothermal treatment. The surface-modified YTZP were characterized by FTIR, AFM and FE-SEM, and then were cocultured with MG-63 osteoblast cells for the biocompatibity assay. The cell morphology and proliferation were evaluated by SEM, WST-1 and ALP activity assay. The XRD results indicated that the phase transformation, from tetragonal phase to monoclinic phase, was increased with increasing the incubation time of hydrothermal treatment. However, no significant differences were successfully grafted onto the YTZP. From the SEM images shown that the MG-63 cells appeared polygonal, spindle-shaped, and attached on the RGD-peptide midified YTZP. The proliferation and cellular activities of MG-63 cells on the RGD-peptide modified YTZP were better than that on the YTZP.
From results of the above, the RGD-peptide immobilized on YTZP surface were improved after surface modification using hydrothermal treatment. The RGD-peptide immobilized YTZP could increase the cell adhesion further improve the biocompatibility of YTZP.
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