The Application of Magnetic Nanomaterials in Peripheral Nerve Conduits

• Main Authors: Hung, Sing-De, 洪興德
• Other Authors: Chang, Chen-Jung
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/99785118390601853732

碩士 === 中臺科技大學 === 醫學影像暨放射科學系暨研究所 === 101 === A trend toward cell tracking by magnetic nanomaterials was performed during these years. However, no study was focused on Schwann cells migration under magnetic field exposure.In this study cultured Schwnn cells were added Gd-DOTA and then a magnetic field drive by magnetic resonance (MRI) were utilized. Schwann cells were cultured in H-DMEM and supplemented with 10% fetal bovine serum；Gd-DOTA was added to the cultured medium. Cells were exposed under magnetic fields and the cells migration. cell viability were recorded. The cells aligned and orientation was also observed by S.E.M and S-100 lmmunostaining. The results of regenerated rat sciatic nerve presented by MRI images displayed the highest scores in 5% Gd-DOTA groups. And the results were similar with the in-vitro experiments.