| Summary: | 碩士 === 中山醫學大學 === 口腔科學研究所 === 101 === Catechins in cancer-related research have received great attention in recent years. The (-)-epigallocatechin-3-gallate (EGCG) are most abundant compound in extracted that effective inhibits the growth of cancer cells. Theaflavins extracted from completely fermented black tea are also inhibits the growth of cancer cells effectively. In this study, we use human colorectal cancer cell lines that were treated with tea extracts such as EGCG or TF-3-G, and their effects on the HCT116 cells were investigated. EGCG or TF-3-G inhibits the growth of HCT-116 cells through triggering the G2/M arrest in HCT-116. We further examined cellular-signaling protein and found that EGCG or TF3-G increase the activities of JNK and also p53 and p21 expression, but reduces Cyclin A and Cyclin B expression. HCT-116 cells pre-treated with JNK Inhibitor (SP600125) before adding EGCG or TF-3-G were examined and we found that EGCG or TF-3-G is able to reverse G2/M arrest in HCT-116, wherein p53, p21, cyclin A and cyclinB protein levels are not significantly changed comparison to cells without treated EGCG or TF-3-G. We found that EGCG or TF-3-G inhibits HCT-116 cells growth through mechanisms that influence on the activity of JNK signaling pathway, and future change cyclin A and Cyclin B expression levels to induce cell cycle arrest.
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