Mulberry leave extracts inhibit type IIdiabetic nephropathy

• Main Authors: Bo-Han Wen, 溫柏瀚
• Other Authors: Horng-Rong Chang
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/88765834325012396898

碩士 === 中山醫學大學 === 醫學研究所 === 101 === Backgrounds: Diabetic nephropathy is a major cause of End-stage renal disease and the mortality rate due to this disease is continuously progressing worldwide. 8 to 10% patients of type 2 diabetes will suffer from diabetic nephropathy. Here, we design this study to elucidate the role of mulberry leave extracts (MLE), a traditional Chinese medicine as an anti-diabetic agent, in the pathogenesis of DN. Materials and Methods: The mouse cell aspect mesangial cell (MES 13 cell line) was purchased from the Food Industry Research Institute Biological Resources Conservation and Research Center. The cellswere treated with the mulberry leaf aqueous extract (MLE) in high glucose condition, andthe inhibitory effect of MLE cell proliferation induced by high glucosewas assayed by Western blot. 100mg/Kg streptozotocin (STZ) and 240mg/Kg nicotinamide (NA) were administered to C57BL/6J mice with high fat diet and 1%, 3% of MLEs. The plasma glucose concentration, body weight, oral glucose tolerance, insulin tolerance and albuminuria were monitored every two weeks. After 8 weeks later, mice were sacrificed and the kidneys were assayed by H.E.,PAS and Masson trichrome staining. Resultsand Conclusion: The results from the oral glucose tolerance (OGTT) and insulin resistance (ITT) tests showed that MLE decreased the plasma glucose and improved the insulin sensitivity in type II mice compared with the native group,indicating that MLEs ameliorated the renal atrophy in DM mice. In addition, diabetes-caused glomerular atrophy was recovered after treatment with MLEs in diabetic mice. Besides, MLE improved the MES-13 cells proliferation and ECM synthesis enhanced by high ambient glucose.Our results demonstrate that MLE has beneficial effects in diabetic nephropathy.