The effect of medical nutrition therapy on the cell number and viability of epithelial progenitor cells derived from neonatal umbilical cord blood in gestational diabetes mellitus women

• Main Authors: Shu-Ling Chang, 張書綾
• Other Authors: 黃怡嘉
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/14963215580244347747

碩士 === 中山醫學大學 === 營養學研究所 === 101 === Gestational diabetes mellitus (GDM) is defined as glucose intolerance with first onset during pregnancy, and will increase the adverse effects of perinatal outcomes. The prevalence of GDM ranges from 1 to 14%. Epithelial progenitor cells (EPCs) involve uterine vasculature remodeling during pregnancy. Hyperglycemia would increase apoptosis and impair the function of EPCs. However, the effects of maternal hyperglycemia and strict diet and weight control on the cell number and viability of fetal EPCs in GDM women are not clear. The aim of this study was to investigate the effect of medical nutrition therapy on the cell number and viability of EPCs derived from neonatal umbilical cord blood in GDM woman. This study was an intervention study. GDM women with gestational age 24 to 32 weeks were recruited in Chi-Mei hospital between September, 2011 and May, 2013. They were divided into the GDM-MNT group or the GDM-routine care group based on their intention. Healthy pregnant women were recruited as the reference group. All women were followed to delivery. The neonatal umbilical cord blood was collected after the delivery. We analyzed the cell number of EPCs derived from umbilical cord blood, and tested the cell viability of EPCs derived from umbilical cord blood by heatstroke animal model. No significant differences were found in maternal characteristics and perinatal outcomes among three groups. However, there were more cell number and viability of EPCs derived from neonatal umbilical cord blood in the non-GDM group compared to the GDM group. The cell number and viability of EPCs derived from neonatal umbilical cord blood in the GDM-MNT group were significantly higher than the GDM-routine care group. Our study showed that medical nutrition therapy can attenuate the cell dysfunction and increase the cell number of EPCs derived from neonatal umbilical cord blood in GDM women.