Study of Human Parvovirus B19-VP1u Proteins on Liver of Balb/c Mice

• Main Authors: Shun-Chih Chang, 張舜智
• Other Authors: Tsai-Ching Hsu
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/88115625755768995675

碩士 === 中山醫學大學 === 微生物免疫研究所 === 101 === Human Parvovirus B19 is a significant human pathogen in Parvoviridae. It has been re-ported that B19 can be detected in blood or liver specimens in patient with liver diseases. Indeed, various studies have postulated a connection between B19 infection and liver injury. Recently, studies suggested that the PLA2 motif of B19-VP1u play an important role in closely associated with B19-infected and inflammatory. However, the precise mechanism is still obscure. In the present study, we aimed to investigate the influence of B19-VP1u in Balb/c mice by injected subcutaneously with COS-7 cells expressing VP1u and observed using a non-invasive IVIS bio-luminescent imaging system. Our experimental results revealed that enhanced MMP-9 activity and increased phosphorylation of p38, ERK, IKK-α, IκB and NF-κB(p65) proteins in liver from Balb/c mice receiving B19-VP1u. Additionally, significantly increased inflammatory associated proteins, including CRP, IL-1β and IL-6 were observed in liver from Balb/c mice receiving B19-VP1u. Notably, phosphorylated STAT1 proteins were involved in the induction of inflammatory cytokines in liver from Balb/c mice receiving B19-VP1u. Our findings revealed the effects of B19-VP1u on liver injury and provide a clue in understanding the role of immuno-pathogenesis of B19-VP1u.