Investigating anti-inflammatory effects of Abelmoschus manihot extract on Lipopolysaccharide-stimulated murine microglia cell BV2

• Main Authors: shan-yu wu, 吳珊瑜
• Other Authors: 高紹軒
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/07623761947269475193

碩士 === 中山醫學大學 === 生化暨生物科技研究所 === 101 === Microglia are resident immune cells in the normal brain and play a crucial role in the innate immune response in the central nervous system，continuous or over acting might be harmful to the central nervous system。Microglia are activated by extracellular stimuli such as lipopolysaccharide , interferon-γ , and β-amyloid and activated microglial cells initiated several major cellular responses that play important roles in the pathogenesis of inflammation。 Previous studies have show that Abelmoschus manihot extract (AME) has demonstrated significant anti-inflammatory activity in kidney disease，thus it was hypothesized that effect of Abelmoschus manihot extract (AME) would suppresses lipopolysaccharide(LPS) induces neuroinflammatory responses in BV2 microglia cell. We used BV-2 microglia cell line to study the anti-neuroinflammatory mechanism of Abelmoschus manihot extract (AME). Abelmoschus manihot extract (AME) at concentrations ranging from 5 to 100 μg/ml did not affect cell viability using MTT assay. LPS increase the production of nitric oxide by sevenfold,however, Abelmoschus manihot extract (AME) effectively antagonized LPS-induced nitric oxide production.and RT-PCR data suggest, Abelmoschus manihot extract (AME) effectively inhibited LPS- induced iNOS, COX-2 expression.and we found AME suppressed the LPS induced phosphorylation of MAPK including ERK1/2、JNK、p38.