| Summary: | 碩士 === 中山醫學大學 === 生化暨生物科技研究所 === 101 === AMP-activated protein kinase (AMPK) is a key player in maintaining energy homeostasis in response to metabolic stress. Published studies indicate that AMPK activation strongly suppresses cell proliferation in
non-malignant cells as well as in tumour cells. This study examined the anti-tumor mechanism of Energi-702 (AMPK activator) on osteosarcoma cell lines (U2OS & 143B). Cell viability was detected by the MTT assay and cell morphology was observed after drug administration. After treated with Energi-702 for 24 hour, osteosarcoma cell lysates were collected. Western blot results demonstrate that cell cycle-associate proteins cyclin D1, cyclin E, cdk 2 and cdk 4 were inhibited after Energi-702 treatment. We also found that tumor suppressor gene p27 and p53 have significant elevation after Energi-702 treatment. On the other hand, the western blot result showed the activation of caspase-3, caspase-9, and PARP by Energi-702. In parallel, Energi-702 significantly decreased the level of anti-apoptotic protein, Bcl-2. Taken together, these findings indicate that Energi-702 diminished cell viability through cell cycle arrest and apoptosis.
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