Cardioprotection and Hepatoprotection of naturally fermented noni (Morinda citrifolia L.) juice in a high fat diet and chronic alcohol consumption

• Main Authors: Yi-Ling Lin, 林怡伶
• Other Authors: 曾博修
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/25234104799858631065

博士 === 中山醫學大學 === 生化暨生物科技研究所 === 101 === It has been summarized that a western-type dietary habit or chronic alcohol consumption easily results in hyperlipidemic, cardiovascular, and liver disorders. Meanwhile, chronic liver disease and cirrhosis are serious diseases facing to either worldwide or Taiwan. One-year naturally fermented noni juice (NJ) contains polyphenols, polysaccharides, and some trace minerals. Hence, this dissertation involved three independent studies on the protective effects of NJ against hyperlipidemia, high-fat-diet induced fatty liver, and alcoholic fatty livers, respectively. In the first study of this dissertation, NJ supplementation decreased (p<0.05) serum triacylglycerol (TAG), cholesterol (TC), atherogenic index, triobarbituric acid reactive substances (TBARS) values, and hepatic lipids in high-fat/cholesterol diet (HFCD) fed hamsters, whereas serum trolox equivalent antioxidant capacity (TEAC), glutathione (GSH), and fecal lipids were increased (p<0.05) by NJ supplementation. Besides, NJ supplementation downregulated (p<0.05) sterol regulator element binding protein-1c in HFCD fed hamsters, but upregulated (p<0.05) hepatic peroxisome proliferator-activated receptor-alpha and uncoupling protein 2 gene expressions in HFCD hamsters. In the second study, after a 6-week feeding period, increased (p<0.05) sizes of liver and visceral fat in high-fat dietary hamsters were ameliorated (p<0.05) by NJ supplementation compared to the normal fat dietary hamsters (Control group). NJ also decreased (p<0.05) serum/liver lipids but enhanced (p<0.05) daily faecal lipid/bile acid outputs in high-fat dietary hamsters. Meanwhile, high-fat dietary hamsters co-treated with NJ enhanced (p<0.05) liver antioxidant capacities but lowered (p<0.05) liver iNOS, COX-2, TNF-α, and IL-1β expressions, and gelatinolytic levels of MMP9 and serum ALT values compared to those without NJ. The last study was to assess the hepatoprotective effects of NJ against a chronic alcohol consumption. NJ supplementation decreased (p<0.05) serum AST, ALT and liver lipids, as well as increased (p<0.05) daily fecal lipid outputs in alcohol-diet fed mice. Besides, NJ supplementation not only downregulated (p<0.05) hepatic lipogenesis but also upregulated (p<0.05) fatty acid-β-oxidation-related gene expressions in alcohol-diet fed mice. Alcohol-diet fed mice cotreated with NJ had higher (p<0.05) hepatic TEAC and GSH levels but lower (p<0.05) TBARS value, TNF-α, and IL-1β expressions compared to those without NJ.