Analysis of the Escherichia coli strains on liver abscess

• Main Authors: Shu-Feng Feng, 馮淑楓
• Other Authors: Bor-Show Tzang
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/70324831945732649350

碩士 === 中山醫學大學 === 生化暨生物科技研究所 === 101 === Recently, many research indicate that the plasmids isolated from Escherichia coli include AmpC β-lactamase gene. The function of AmpC is resistant against the β-lactam antibiotics, just the same as the other enterobacteriaceae. Because β-lactam resistant Escherichia coli also resistant against cephamycin and β-lactam inhibitor antibiotics. Thus, it can different from Extended-Spectrum Beta-Lactamases ( ESBLs ). In this study, the Escherichia coli strains collected from the bacteria lab department of pathology National Cheng- Kung university hospital. The collect interval is AD 2002-2010. Total 24 Escherichia coli strains isolated from liver abscess patients. Testing by Double disk synergy test and Disk diffusion confirmatory test. The result is Escherichia coli ( ESBL ) phenotype ratio is 8.3% (2/24). Then analyze genes associated Escherichia coli metabolic enzymes by Polymerase chain reaction ( PCR ). Result: The ratio of TEM type is 45.8% (11/24). The ratio of CMY-2 type is 20.8% (5/24). The ratio of CXM type is 12.5% (3/24). By sequnecing﹐blaCTX-M have 3 subtypes including blaCTX-M-3、blaCTX-M-14、blaCTX-M-15. The most common is blaCTX-M-3 and blaCTX-M-14. The phylogenetic typing of 24 strains can divided into four types: A, B1, B2 and D. Farther, typing by Pulsed-field gel electrophoresis ( PFGE ) and Multilocus sequence typing ( MLST ), the 24 strains can divided into 23 PFGE types and 16 MLST types. In this study indicate that Escherichia coli with AmpC β-lactamase isolated from liver abscess could type into ESBL、TEM typing、CTX-M typing and CMY-2 typing.