| Summary: | 碩士 === 嘉南藥理科技大學 === 保健營養系 === 101 === Background and Purpose: Diabetes mellitus (DM) is a chronic metabolic disease. Obesity is a risk factor for type 2 DM and also is linked with cardiovascular disease and chronic kidney disease. Gynura formosana and Litchi chinensis seed has been shown to possess many beneficial health effects, including liver protection, anti-oxidation, anti-cancer and anti-inflammation. But little is known about the effect of Gynura formosana and Litchi chinensis seed on diabetes. This study was to examine whether Gynura formosana and Litchi chinensis seed show anti-diabetic effect in mice fed with high-fat and high-sucrose diet. Methods: Five groups of male C57BL/6j mice were fed for 10 weeks with a high-fat and high-sucrose diet (35% fat and 35.9% sucrose, HSF), a HSF diet with 0.1% or with 0.5% Gynura formosana extract (G1, G5) and a HSF diet with 0.1% or with 0.5% Litchi chinensis seed extract(L1, L5). The body weight, tissues weight, the concentration of plasma glucose, insulin, adiponectin, lipids, liver lipids, urine BUN and creatinine were assayed after 10 weeks. Results: The body weight, the relative weight of epididymal fat and retroperitoneal fat, the liver cholesterol and urine creatinine levels in G5, L1 and L5 groups significantly decreased when compared to HSF group. In G5 and L5 groups, the levels of plasma insulin, the HOMA-insulin resistance were significantly lower than that in HSF group. The G5 groups showed significantly lower plasma triglyceride level than that in HSF group. However, the plasma adiponectin levels in G5 group showed 1.2-fold higher than that in HSF group. The L1 group showed significantly lower plasma glucose, HOMA-insulin resistance, kidney TNF-α, triglyceride and muscle GLUT-4 than that in HSF group. Conclusions: We conclude that a high-fat and high-sucrose diet increased body weight, adipose tissue mass, and plasma insulin in mice and these effects can be reduced by the water extracts of Gynura formosana and Litchi chinensis seed. Gynura formosana and Litchi chinensis seed extract might have the diabetic prevention effect in obese mice induced by high-fat and high-sucrose diet.
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