| Summary: | 碩士 === 嘉南藥理科技大學 === 化粧品應用與管理系 === 101 === In recent years, the overproduction of citrus fruits has resulted in an unnecessary increase in agricultural wastes in Taiwan. In an attempt to find an application for these potentially valuable wastes, we evaluated the antioxidant and whitening properties of Citrus grandis Osbeck. The ethanolic extract, 95% MeOH fractions from dried C. grandis peel revealed strong 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity. Inhibition of ABTS, inhibition LOX activity, Chelating effect and tyrosinase inhibition.The n-hexane and 95% MeOH layer were purified by a combination of silica gel chromatography and Sephadex LH-20. Eight compounds were isolated, where Naringin (1, 582.2 mg), Limonin (2, 356.6 mg), Meranzin hydrate (3, 2.86 g), -Sitosterol (4, 177.9 mg), Isoimperatorin (5, 179.8 mg), Stigmasterol (6, 427.9 mg), Bergaptol (7，119.2 mg), -Sitosterol-D-glucoside (8, 114.6 mg). Their structures were established on the basis of spectral evidence.
From Citrus grandis Osbeck was isolated compound 7 showed DPPH scanvenging activity and compounds 2 and 7 showed inhibition of ABTS activity and compounds 2, 5 and 7 showed inhibition of LOX activity. The compounds 1, 2 and 3 were confirmed to inhibit tyrosinase.
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