| Summary: | 碩士 === 嘉南藥理科技大學 === 化粧品應用與管理系暨化粧品科技研究所 === 101 === The purpose of this study was to evaluate the effects of anti-oxidation, anti-inflammation, anti-microorganism and cytotoxicity in flowers of different colors from Ixora and other flowering plants, then they were extracted with boiled water. The kinds of plants were Ixora × williamsii ‘Sunkist’, Ixora × williamsii ‘Dwarf Pink’, Ixora × williamsii ‘Dwarf Alba’, Ixora duffii ‘Super King’, Duranta repens Linn. and Lantana camara Linn. Extracts of these flowers were prepared by hot water, and lyophilized into dry powders to evaluate the series of biological activity.
For the anti-oxidation activity assay, the extracts of I. × w. Dwarf Yellow showed the best antioxidant capacity, then the total phenolic content was 194.60 ± 0.63 mg GAE/g extract; the total flavonoid content was 168.45 ± 0.63 mg CE/g extract; for the scavenging effect of DPPH•, the EC50 was 23.12 ± 0.45 μg/mL; for the reducing power capacity test, the EC50 was 17.35 ± 0.85 μg/mL; for the Trolox equivalent total antioxidant capacity assay, the Trolox content was 2.204 ± 0.01 mmol TE/g extract; for the inhibition of lipid peroxidation assay, the extracts of I. × w. Dwarf Pink showed the best result, and the inhibition rate was 88.85 ± 0.48%; but on the study of ferrous ion chelating ability, all the sample’s EC50 value were above 3 mg/mL. For the anti-inflammatory activity assay, by the HA-zymography method, all the samples could inhibit the HAase activity, except D. repens and L. camara.The antimicrobial activity was determined by paper disc diffusion assay, in anti-Gram-positive bacteria, I. × w. Sunkist displayed the highest inhibitory effects against Staphylococcus aureus, and the inhibitory zones was 12.67 ± 0.58 mm, then I. × w. Dwarf Yellow showed the highest inhibitory effects against Bacillus subtilis, the inhibition zone was 13.17 ± 0.29 mm; in anti-Gram-negative bacteria, I. Super King displayed the highest inhibitory effects agains Escherichia coli, and the inhibitory zones was 10.50 ± 0.50 mm, then L. camara showed the highest inhibitory effects against Pseudomonas aeruginosa , the inhibition zone was 10.17 ± 0.76 mm; however, the anti-microbial activity in yeast, all the samples were no inhibitiory ability against Candida albicans; on the MIC and MMC test, the concentration of extract was above 1000 μg/mL. Finally, for the cell viability test, I. × w. Dwarf Pink and I. × w. Dwarf Alba had no cytotoxicity, and I. × w. Dwarf Alba seemed to be able to promote the cell proliferation; while I. × w. Sunkist, I. × w. Dwarf Yellow, I. Super King and D. repens had low cytotoxicity; although it is low cytotoxicity in L. camara, when extract’s dose increased and the cells would decrease the survival rate.
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