| Summary: | 碩士 === 嘉南藥理科技大學 === 生物科技系 === 101 === Prior literature suggests that D. radiodurans (Deinococcus radiodurans, Dr) after UV irradiation, and then add the manganese Mn (II) may induce some cultured protein expression, so that repair damaged cells and has been the phenomenon of secondary growth the complexity of the repair mechanism is very worth exploring. Carotenoids which protection is also an important key; these carotenoids protect them from UV and radiation damage of the factors.
In this study, D. radiodurans to UV irradiation Add Sini- Tang culture, then observe and compare the added Mn (II) after culture differences.
The first experiment was observed by scanning electron microscopy extracellular damage situations; Then extract the red pigment, Thin Layer Chromatography (TLC) and Liquid Chromatography-Mass Spectrometry (LC / DAD-MS) detection of their performance and changes and using Electrospray Ionization / Atmospheric Pressure Chemical Ionization / Laser Desorption ionization (ESI / APCI / LD-MS) were identified; finally re-use Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometer analysis of these red pigments and proteins performance differences.
The results show that Deinococcus radiodurans, Dr appearance and can not judge the damage, the degree of recovery to TLC analysis the extracted red pigment is a significant change, the LC / DAD-MS found at 400-500nm with a small amount of absorption, but MS is not related to the measured signal. Further use of ESI / APCI / LD-MS, can be detected m / z 567 (M + H) + signal, via MS / MS to determine which compound was identified as 2-deoxydeinoxanthin. Analysis of Deinococcus radiodurans, Dr to UV damage added after Mn (II) and Sini-Tang cultivation of protein after extraction through principal component analysis clearly shows its difference.
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