| Summary: | 碩士 === 嘉南藥理科技大學 === 生物科技系 === 101 === Fungal secondary metabolism is very important in fungal growth and development. The production of secondary metabolites is relied on the changes of environment. In Aspergillus, gene cluster is involved in the regulation of secondary metabolism, and the major products of the gene cluster are belong to non-reducing polyketide synthases (NRPKSs). However, it is not very clear about the function and activity of the secondary metabolites catalyzed by NRPKSs. Cordyceps militaris is a useful herbal medicine grew on some insect larvae or pupae. Cordyceps militaris contains many kinds of functional components such as cordycepin, cordycepic acid, nucleosides, and Cordyceps polysaccharide. We assume that the secondary metabolic pathway is also existed in C. militaris. In order to clarify the function of NRPKSs in the regulation of secondary metabolism and the secondary metabolites in C. militaris, fusion PCR technique was used to the recombination of selective markers , alcA promoter, and target gene. Heterologous expression was performed in Aspergillus nidulans. In this study, the use of fusion PCR completed which NRPKS fusion gene, and seven of transformants were obtained. The variety of secondary metabolites was analyzed by high-performance liquid chromatography. The results indicated that some secondary metabolites were produced in transformants. This study showed a great potential for developing the application of fungal secondary metabolites and studying the function of NRPKS genes.
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