Inhibitory effects of DBL from Phellinus linteus on LPS-induced inflammatory response in RAW264.7 macrophages

• Main Authors: Ssu-Wen Wang, 王思雯
• Other Authors: Guan-Jhong Huang
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/29330992817441275627

碩士 === 中國醫藥大學 === 中國藥學暨中藥資源學系碩士班 === 101 === Excessive or uncontrolled inflammation has been confirmed and progression of many diseases, including: rheumatoid arthritis, multiple sclerosis, and neurodegenerative diseases such as a special characterization. Therefore, many experiments dedicated for searching food ingredients witch can exert inhibition of over-inflammation and have a minimum of side effects of the ingredients. Phellinus linteus is a highly cost-effective health food material, has been confirmed with the anti-cancer, anti-inflammation and anti-allergic effect. The experiment will explore the polyphenolic natural compounds from Phellinus linteus in DBL. And we use DBL on LPS-induced inflammatory response in RAW 264.7 macrophage cell line. We found DBL didn’t affect the cell viability of RAW 264.7 cell. Moreover, DBL would inhibit the COX-2 and iNOS protein expression, and when given DBL can significantly reduce the intracellular content of TNF-α and IL-1β in a does dependent. Besides, we further observed intracellular signaling molecules, containing phosphatidylinositol 3-Kinase (PI3K)-AKT and mitogen activated protein kinase (MAPK) pathway. And we found DBL inhibited the inflammation via the PI3K/Akt pathway and MAPK family ERK1/2 and c-JNK, p38 protein expression. NF-κB activity is dominated by its inhibiting molecular IκB and upstream signal IKK. Therefore, we also explore IKK/IκB/NF-κB pathway. And our data indicate DBL could suppress IKK and IκB phosphorylation, and increase the NF-κB protein expression in cytoplasm. Based on the above experimental results, the data indicate that DBL could inhibit LPS induced inflammatory response in vitro. And DBL has capability to develop as an anti-inflammatory newborn drug in the future.