Development of high-throughput pneumatically-driven perfusion micro 3-dimensional cell culture system and the study of the effect of primary cancer cell culture models on the result of chemosensitivity assays of anti-cancer drugs.

• Main Authors: Yi Dao Chen, 陳以道
• Other Authors: M. H. Wu
• Format: Others
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/91807634568958629057

碩士 === 長庚大學 === 生化與生醫工程研究所 === 101 === Traditional cell culture devices are widely used in cell culture-based testing (eg: drug testing). However, most of the cell culture tools may not be able to provide a stable, quantitative physiological significance possessed cell culture environment for high-throughput and high accuracy of the drug testing. Therefore, this study aims to develop a miniaturized three-dimensional cell culture perfusion system. The cell culture system main features are: (a) to gas-driven approach to multi-channel transmission medium and (2) the efficient conduct highly accurate small sample (example: three-dimensional cell culture samples) injection. Development section of the device, we polydimethyl siloxane as the material production of the cell culture system, in addition we also evaluated the gas-driven multi-channel medium conveying performance. Finally, we will demonstrate in the original cell culture. On the application in terms of the ultimate vision of this research is the use of cell culture systems developed by anticancer drug sensitivity testing in the hope that the future can provide patients with cancer chemotherapy drug choice of targets. In achieving this goal, some of the cell culture test results for such usage patterns affect basic research will be established. Experimental results show that the Institute is the development of the gas-drive system uses gas operation time and the intervals to control the perfusion flow rate at the action time is greater than one second when the coefficient of variation can reach 2.32% to 1.20% and the culture environment pH reaches stability, and the original cancer cells in different cell culture environment pH value below its physiological performance and growth capabilities were significantly different, so the system in providing stable homogeneous cell culture environment, with good performance. The use of this system for further experimental results also show that the original cancer cells in different cell culture models under its physiological performance and growth are significant differences, while affecting the original cancer cells to anticancer drugs chemical sensitivity, and therefore relevant in vitro experiments, which must be considered and the environment into the cell culture model is selected to obtain a physiological significance of the experimental results.