High-efficiency co-immobilized microorganism culture system using loofah sponge for ethanol production from rice straw in a modified co-culture cell bioreactor

• Main Authors: Po Min Lien, 連博民
• Other Authors: Y. K. Liu
• Format: Others
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/48894884981852204350

碩士 === 長庚大學 === 生化與生醫工程研究所 === 101 === In recent years , cellulosic ethanol is the one of renewable energy that can replace tranditional petrochemical fuel . Agricultural wastes contains large amounts of cellulose , hemicellulose , lignin and other components , cellulose and hemicelluloses can transform to glucose and xylose by hydrolyzing . Furthermore , it can produce bioethanol . The initial flask experiments , it shows that the ethanol yield is 3.18 g / L , the conversion rate was 62.4% when the rotention speed is 75 rpm , the ratio of Z. mobilis and P. stipitis is 1: 3 , and separated-immobilization of fermentation conditions . Then , we use rice straw as substrate , to discuss aeration rate, stirring speed and other factors , expect to get the best ethanol yield and metabolic rate . The experiment will be simultaneous saccharification and co-fermentation (SSCF) in reactor to investigate the high substrate concentrations and repeated batch . At present , the initial concentrate in addition to the 10 g / L rice straw , we also discuss 20 g / L and 30 g / L . The results show that the initial substrate concentration is higher , the lower the rate of ethanol conversion . And in the experiment ,we found that the substrate concentration of 30 g / L, the bottom of the aerobic zone caused by congestion due to rice straw powder , this problem will be used repeated batch culture to improve .In hydrolyzate discuss, we add 10 g/L rice straw as raw material which the medium after over-liming , the ethanol yield can be achieved 814 ppm. After then, the repeated-bacth fermentation medium