| Summary: | 碩士 === 長庚大學 === 生化與生醫工程研究所 === 101 === The purpose of this study is to investigate effect of dynamic compression magnitude and frequency to the physiology of bovine articular chondrocytes. The research is uses a high throughput perfusion micro three-dimensional cell culture system capable of providing dynamic compressive stimulation which developed by our laboratory previously, and embed bovine articular cartilage in alginic acid to form three-dimensional cell / hydrogel structures, then applied pneumatic pressure causing elastic deformation to impose on the three-dimensional cells / hydrogel structures with different stimulation intensity (0%, 20% and 40% compressive strain) and stimulation frequency (0.5Hz, 1Hz and 2Hz). In the end, evaluate the affection of dynamic compression stimulation on bovine articular cartilage physiology by measuring DNA content, the production rate of lactate, glycosaminoglycans and hydroxyproline. The results disclosed that under the same stimulation frequency conditions of 1Hz ×(0%, 20%, 40%) and 2Hz ×(0%, 20%, 40%), bovine articular chondrocytes lactate production rate enhanced at 40% compression magnitude, and both 20% and 40% compression magnitude increased glycosaminoglycans producing. Under the same magnitude stimulation conditions of 20% ×(0.5Hz、1Hz、2Hz) and 40% ×(0.5Hz、1Hz、2Hz), All stimulation frequency higher than 1 Hz (included) had promoted the formation of glycosaminoglycans, and at 40% ×2Hz stimulation conditions improved the formation of lactate shows increasing. However, the proliferaion of bovine articular chondrocytes and production rate of hydroxyproline was not affected by any compression stimulation.
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