Characterization of Drosophila CG31235 mutant

• Main Authors: Chia Yu Tai, 戴嘉俞
• Other Authors: L. M. Pai
• Format: Others
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/26302550745193289069

碩士 === 長庚大學 === 生物醫學研究所 === 101 === Short-chain dehydrogenases/reductases (SDRs) constitute a large family of NAD(P)(H)- dependent oxidoreductases. Enzymes of SDR family have critical roles in lipid, amino acid, carbohydrate, cofactor, hormone and xenobiotic metabolism. According to the Conserved Domain Database analysis of CG31235, we suppose that it is a retinol dehydrogenase which belongs to SDR subfamily. CG31235 mainly expresses in embryo stage and its transcript is expressed the longitudinal (interface) glial cells. The function of this gene in Drosophila development is unknown yet. By P-element excision, we generated five CG31235 mutant alleles. To find out the defects in CG31235 mutant, we examined nerve system and glial cell patterns using specific markers in comparison of wild-type and mutants. However, immunostaining assay indicated that there is no structural defect in the nerve system . Furthermore, we observed that CG31235 mutant larvae displayed a severe impairment in movement after hatch. And these larvae stopped moving then died in the early instar larval stage. Accordingly, we assayed CG31235 mutant larvae behavior and found that these mutants showed dramatically reduced peristaltic contractions numbers and crawling distance compared with wild type . During chromosome analysis, we found that CG18617 expression was also reduced in our mutant lines. We supposed that mutant death may caused by CG18617 since it was an subunit of vacuolar proton ATPase that influenced cell pH. And CG31235 may have some effects in Drosophila development late stage.