| Summary: | 碩士 === 長庚大學 === 生物醫學研究所 === 101 === Magnetic nanoparticle (MNP) has been used as drug carriers in targeting therapy, thus MNP uptake by cells may be critical for drug reaching its intracellular target. Previous studies indicated that magnetic force and surface characteristics of MNP affected cellular uptake and intracellular localization of MNP. We hypothesized that MNP surface can be modified with chemical compounds, affecting its interaction with plasma membrane, and influencing endocytosis pathway. Quantitative and qualitative results of human glioma cells (U87MG) uptake of MNP were obtained by potassium thiocyanate method and transmission electron microscopy. The results shown that magnetic force, epigallocatechin gallate (EGCG) and poly-L-lysine (PLL) enhanced cellular uptake of MNP in a concentration- and time- dependent manner. The enhancing effects of EGCG and PLL may be through interacting with MNP and plasma membrane. Dextran-coated MNP modified with PLL enhanced cellular uptake in a concentration- and time-dependent manner. EGCG attenuated cellular uptake of PLL-modified MNP in certain conditions, indicating the enhancing effect of EGCG and PLL may act on a similar mechanism through interaction between MNP and plasma membrane. Cellular uptake of MNP associated with the zeta potential and coating polymer of MNP. The results suggested that MNP surface characteristics are critical on cellular uptake; the enhancing effects of EGCG and PLL may involve in a similar mechanism, which is potentially amenable to future therapeutic application in treatment of glioma.
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