| Summary: | 碩士 === 國立中正大學 === 生物醫學研究所 === 101 === SOX2 (Sex-determining region Y (SRY)-box protein 2) is a member of the HMG transcription factor family, functioning as a transcription factor and involving in cell self-renewal, differentiation, proliferation and apoptosis. Over-expression of SOX2 has been demonstrated in a least 60 cancer cell types. The study conducted in our laboratory indicated that SOX2 also regulates alternative splicing in bladder cancer, implicating that SOX2 is not only a DNA transcription factor but also as a RNA binding protein. In my study, I intend to determine the preferred binding sequence of SOX2 by employing oligomer-directed RNase H digestion-coupled CLIP assay. To demonstrate whether SOX2 direct regulated mRNA, We first expressed SOX2 and E1A in BFTC905, follow by splicing reporter CLIP assay. The data suggest SOX2 directly binding on E1A. Furthermore, we determined that SOX2 regulates splice site selection of E1A, indicating that SOX2 is a bona fide splicing factor. Furthermore, the binding sequence of SOX2 on the S100A14 mRNA also determined through similar approach. Those data suggest that SOX2 regulates translation of the S100A14 mRNA by direct binding on the
S100A14 mRNA
Keywords: CLIP、 SOX2、 RNA binding protein
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