Human Adipose-derived Stem Cells as the Vehicles of Therapeutic Retrovirus for Treating Glioma

• Main Authors: Chiu, Yiting, 邱怡婷
• Other Authors: Tai, Chienkuo
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/66819507359689422090

碩士 === 國立中正大學 === 分子生物研究所 === 100 === Glioma accounts for the majority of primary malignant brain tumors and remains virtually incurable despite extensive surgical resection, radiotherapy, and chemotherapy. Glioma is difficult to treat because it has an exceptional infiltrative potential and proclivity to integrate into normal brain tissue. Recently, stem cells have been found offering much promise as delivery vehicles for brain tumor therapy. We hypothesized that human adipose-derived stem cells (hASCs) possess the ability to deliver therapeutic virus vectors to tumor sites. In this study, we examined the hypothesis in U87 by using hASCs infected with a replicating retrovirus vector (RRV) containing green fluorescent protein（GFP）. The result shows that GFP expressing RRV released from the hASCs could further infect glioma cells, achieving tumor-selective and persistent gene delivery to tumors. We have also tested the tumor-tropic migratory ability of hASCs and investigated the efficiency of hASCs to deliver RRV to glioma cells in vitro and in vivo. Next，We evaluated the therapeutic effects of hASCs-ACE-CD (cytosine deaminase) in MTT assay. Finally we also evaluated the therapeutic ability of hASCs-ACE-CD in an orthotopic mouse model of human glioma. We wish the use of hASCs as the vehicle for dispersing RRV may provide a viable strategy for delivering a therapeutic payload to disseminated glioma burdens.