| Summary: | 碩士 === 元智大學 === 化學工程與材料科學學系 === 100 === In human, angiogenesis is not only important for physiology and development, but also correlation with most cardiovascular diseases such as myocardial infraction. Recently, many reports further demonstrated that angiogenesis related closely with tumor development. Angiogenesis and vasculogenesis are regulated by the proliferation and differentiation of endothelial progenitor cells (EPCs).
In this study, we isolated mononuclear cells (MNCs) from human umbilical cord blood and cultured MNCs in the EGM-2 medium. According to the results, several cytokines, especially VEGF165, b-FGF, HGF and IGF-1, have been identified as essential factors to proliferation.
In addition, throughout culture cells preserved their functional capacity that was demonstrated by high uptake of DiI fluorescently conjugated- acetylated-low density lipoprotein (DiI-Ac-LDL), and in vitro tube formation. Flow cytometry demonstrated that EPCs expression of endothelial progenitor markers was not affected by long term in vitro culturing, such as CD31, CD34 , CD105, CD144, CD309 and vWF .
Our results showed that EPCs derived from human umbilical cord blood sample exhibited both characteristics and functions of EPCs. Therefore, we believed EPCs expansion medium would generate large amounts of functional EPCs.
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