| Summary: | 碩士 === 國立陽明大學 === 生物醫學影像暨放射科學系 === 100 === Purpose:
Middle cerebral artery occlusion (MCAO) in rodents is an experimental model of cerebral ischemia that is essential to in vivo pathophysiological processes and is widely used for the evaluation of treatment efficacy after brain injury. In this study, the MCAO in rodents were applied to analyze kinetics with micro positron emission tomography (microPET) when the middle cerebral artery was reopened to observe the different pharmacokinetics (PK) values.
Materials and methods:
Sprague-Dawley rats were anaesthetized with chloral hydrate and subjected to 60 min of intraluminal middle cerebral artery occlusion with subsequent reperfusion, in three groups: control group, observed 4 hrs after MCA reopening group (4h-MCAO) and observed 24 hrs after MCAO reopening group (24h-MCAO ). For all groups, microPET imaging was used to evaluate brain glucose metabolism. The imaging data acquired from microPET were displayed, analyzed and coregistered with built-in MRI template by PMOD 3.2. Metabolism was calculated by drawing ROIs in the bilateral cortex for each slice and then calculated FDG kinectic parameters were calculated.
Results:
After 4h-MCAO, the ipsilateral cortex region of uptake is higher than contralateral cortex region by 21~31%. However, the ipsilateral cortex region of uptake was lower than contralateral cortex region by 31~39% after 24h-MCAO, in which pharmacokinetic parameters in ipsilateral cortex region are also lower than contralateral cortex region.
Conclusion:
In this study, we find that using FDG with microPET scan is effective in detection of metabolic changes and esrimation of PK values of FDG when the MCAO is reopened 4hrs and 24hrs later. In the near future, using medications or instrument involved could use this study as a reference to design the treatment protocol to reduce the affected region in rodents.
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