The role of PKC isoforms in IL-1β-induced myosin light chain kinase expression

• Main Authors: Jia-Hau Chu, 褚家豪
• Other Authors: Hwai-Shi Wang
• Format: Others
• Language: en_US
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/32684556223934912406

碩士 === 國立陽明大學 === 解剖學及細胞生物學研究所 === 100 === Mesenchymal stem cells have been reported that homing to the site of injury, which further lead repair, by inflammatory factors. From our previous study, we found the inflammatory cytokine: interleukin-1β (IL-1β) stimulates mesenchymal stem cell migration by up-regulating myosin light chain kinase (MLCK) expression. PKC/MEK/ERK/NF-κB signaling transduction pathway is involved in IL-1β-induced MLCK expression, but the mechanism has not been fully understood. In this study, we show that IL-1β induces PKCα/βI/δ isoforms, MEK/ERK, and NF-κB signaling activation. By pretreatment with PKC inhibitors: Calphostin C, Go 6976, PKCβ inhibitor, Rottlerin on IL-1β-induced MEK/ERK phosphorylation, we found that PKCα acts upstream of MEK/ERK activation in IL-1β stimulation. Moreover, pretreatment with PKC inhibitors, MEK inhibitor U0126, ERK inhibitor PD98059 on IL-1β-induced IκBα phosphorylation, we found that PKCδ acts upstream of NF-κB signaling activation in IL-1β stimulation. We also demonstrated that NF-κB subunit p65 translocates to nucleus in IL-1β stimulation. In conclusion, IL-1β-induced mesenchymal stem cells NF-κB signaling activation is dependent on PKCδ and then NF-κB subunit p65 translocates to nucleus to up-regulate it’s expression.