Microarray and Deep Sequencing analysis revealed endothelial progenitor cells enriched genes contributed in stemness or angiogenic activities

• Main Authors: Tse-Shun Huang, 黃則舜
• Other Authors: Hsei-Wei Wang
• Format: Others
• Language: en_US
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/64887148686732413533

博士 === 國立陽明大學 === 微生物及免疫學研究所 === 100 === is endothelial progenitor cell (EPC). In the second part, we compared attached EPC (appeared early or late on the culture dish) transcriptomes to those of stem cells and matured endothelial cells. The angiogenic features of late EPCs were reflected by enrichment of vascular development and cell motility genes. Small RNA sequencing provided EPC miRNome patterns that were composed of both novel and known miRNAs. Known pro-angiogenic miRNA such as miR-31 were enriched in late EPCs, and its target FAT4 and TBXA2R were demonstrated to participate in the migratory phenotype. miR-720, which was upregulated in late EPCs after far infrared treatment, was also abundant in late EPCs. Overexpressing or knocking down miR-720 confirmed its pro-angiogenic function. Vasohibin 1 (VASH1), an endogenous angiogenesis inhibitor, was a direct target of miR-720 and represented a major inhibitory target for miR-720 to induce angiogenesis. miR-31 not only targeted FAT4 and TBXA2R, but also regulated miR-720. Our results showed that EPCs manipulate interconnected miRNA-mRNA networks to regulate angiogenic features. Manipulating the expression of angiogenic genes/miRNAs in malfunction EPCs will help to develop novel therapeutic modalities targeting ischemia-related diseases and tumor angiogenesis.