Regulation of Histone deacetylase 3 and Sentrin/SUMO-Specific Protease 1 by miR-X

• Main Authors: Song-Yuan Chen, 陳松遠
• Other Authors: Kou-Juey Wu
• Format: Others
• Language: en_US
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/21848108013291444532

碩士 === 國立陽明大學 === 生化暨分子生物研究所 === 100 === MicroRNAs are endogenous, single-strand RNA molecules that regulate gene expression. MicroRNAs have been identified in different species such as Drosophila, mouse and human. MicroRNA genes located within introns, exons or intergenic areas were transcribed by RNA polymerase II or RNA polymerase III. MicroRNAs are important gene regulators that control cell growth, cell proliferation, cell differentiation and cell death. Recent results have shown that microRNA mutation, amplification, deletion or epigenetic silencing was correlated with various human diseases and cancers. Therefore, microRNA may function as tumor suppressor genes or oncogenes. Epithelial-mesenchymal transition is an important process in cancer metastasis which promote cell migration and cell invasion in cancers. We were interested in identifying novel microRNA(s) involved in epithelial-mesenchymal transition that were induced by hypoxia. From two different predict programs miRBase, TargetScan, the analysis showed that miR-X could target to EMT-relative gene HDAC3 and SENP1. Our preliminary data showed that miR-X could repress luciferase activity of 3’UTR of HDAC3 and SENP1, and the expression of HDAC3 and SENP1 were also repressed in miR-X overexpressing stable lines. miR-X also repress cancer cells migration and invasion ability. For this proposal, I will investigate how hypoxia regulates miR-X expression, and determine how miR-X inhibits the expression of HDAC3 and SENP1. The expression level of miR-X will also be conflated with breast cancer samples to test its prognostic value to predict patients’ survival.