| Summary: | 碩士 === 國立陽明大學 === 生化暨分子生物研究所 === 100 === Bacillus subtilis may encounter various stresses in the environment, such as heat shock, osmotic stress, and starvation for oxygen or nutrients. Therefore a number of adaptive systems have been developed to respond to such stresses. To cope with a sudden temperature upshift, B. subtilis increases the levels of a group of heat shock proteins, including chaperones, and proteases to refold or degrade misfolded proteins. At least six classes of heat shock genes have been identified so far in B. subtilis. The htpG gene is a member of the class IV heat shock genes. In this study, we have found that htpG expression is under the negative control of sigB. We have also found that a highly conserved sequence in the promoter region of htpG is critical for heat induction of htpG. This highly conserved sequence is also present in the promoter regions of some class VI heat shock genes and is crucial for their heat induction. Through the highly conserved sequence present in the promoter regions, we have identified a group of heat-inducible genes relevant to competence, cell division, and osmotic stress. Most importantly, through the highly conserved sequence, we can predict the heat inducibility of some uncharacterized genes in the future.
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