Production of Neofructooligosaccharide by Batch Culture of Xanthophyllomyces dendrorhous

• Main Authors: Yan-Jen Chen, 陳燕貞
• Other Authors: Dey-Chyi Sheu
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/rd24qb

碩士 === 大同大學 === 生物工程學系(所) === 100 === The neofructooligosaccharide (neo-FOS) produced by culture of Xanthophyllomyces dendrorhous (X. dendrorhous) using sucrose as substrate, can stimulate the intestinal microflora. During the fermentation of X. dendrorhous, zinc ion and nitrogen source as well as pH value of the culture broth strongly affect the cell grow and the yield of neo-FOS. Maximal neo-FOS (145 g/L) was obtained when the fermentation was carried out at pH 7 for 48 h, using 20%(w/v) as substrate. Addition of malt extract (3 g/L) enhanced the cell growth. When 30%(w/v) was used, addition of zinc sulfate heptahydrate (0.05 g/L) resulted in a higher amount of glucose compared with that without zinc ion, leading to a lower purity of neo-FOS. Culture with yeast extract (10 g/L) had faster cell growth than that with peptone (5 g/L). However, faster cell growth resulted in a lower purity of neo-FOS. Compared with stirred tank culture, air-lift tank culture produced more neo-FOS (165 g/L) and less amount of glucose, resulting in the highest purity of neo-FOS.