EXTRATION OF OSTRICH OIL WITH SUPERCRITICAL FLUID CARBON DIOXIDE TO ENHANCE THE TRANSDERMAL DELIVERY OF PHYTOSPHINGOSINE

• Main Authors: Niann-Chyi Huang, 黃念齊
• Other Authors: Chung-Yih Wang
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/81186278628092238336

碩士 === 大同大學 === 生物工程學系(所) === 100 === The breeding Taiwan ostrich is mainly for providing meat. The side products such as eggs, feather and skin are also valuable, but the subcutaneous fat was usually abandoned. Ostrich oil is rich in unsaturated fatty acids. Organic solvent extraction or heating method were used to extract ostrich oil from fat. The residual organic solvent and have bad flavor make those methods less popular. In this research, several extraction methods were used to extract ostrich oil. Among those extraction methods, supercriticial carbon dioxide extraction yields the highest amount of unsaturated fatty acids. The condition of 65 ℃ 200 bar have the great yield in small（32 ml extractor） and scale up （100 ml extractor）extraction（74.14±2.07 ％ and 23.87±1.59 ％）. The poor solubility in scale up extraction may result in low yield of oil. After deacidification and deodorization, the oleic acid content can reach as high as 30 ％. The extraction ostrich oil （1 g） was formulated by adding Tween 80 （0.5 g） and Span 60 （0.5 g） and emulsified. Particle size of the ostrich oil cream’s particle size was approximately 600 nm. The capability of increased water content and decreased transepidermal water loss （TEWL） of the epidermis for ostrich oil cream is better than squalane cream. For the transdermal delivery, the permeability coefficient of transdermal delivery for the phytosphingosine encapsulated by ostrich oil cream was 0.32±0.194 cm2/hr, while the permeability coefficient of phytosphingosine which dissolved in ethanol was only 0.246±0.239 cm2/hr. The results suggest that, ostrich oil cream is better than ethanol formula for phytosphingosine transdermal delivery.