| Summary: | 碩士 === 大同大學 === 生物工程學系(所) === 100 === Recently there has been an increasing interest in developing biodiesel as an alternative fuel due to the shortage of fossil fuels and environmental concerns. The advantages of using lipases as biocatalysts for biodiesel production included mild reaction conditions, easy recovery of glycerol and environment friendly. The lipase with activities of hydrolysis, esterification, transesterification, and enantioselectivity has been used in several industries such as food, textile, cosmetic, pharmaceutical, and environmental treatment. In this study, the optimal medium composition for lipase production by Burkholderia sp. was determined by one-factor-at-a-time (OFAT) method and response surface methodology (RSM), respectively. By OFAT, olive oil was the optimal carbon source among tested carbon sources, and 0.5% olive oil could get maximal lipase activity. Tryptone was the optimal organic nitrogen. When 1.5% tryptone was added, maximal lipase activity was found. Ammonium acetate as inorganic nitrogen source, was better than sodium nitrate, ammonium sulfate, ammonium chloride, potassium nitrate and diammonium hydrogen phosphate for lipase production, and the maximal lipase activity occurred when 0.05% ammonium acetate was added. The production of lipase increased with addition of 0.1% Tween 80. 0.05% Magnesium sulfate increased the production of lipase, while zinc sulfate decreased. The maximum activity of 122.3 U/ml was noted by using RSM when Burkholderia sp. was cultivated in the medium composing 0.65% olive oil, 2.42% tryptone and 0.15% Tween 80. The maximum lipase activity determined by RSM increased ca. 17% of that by OFAT.
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