| Summary: | 博士 === 淡江大學 === 化學學系博士班 === 100 === Part Ⅰ: Iron(Ⅲ) Catechol 1,2-Dioxygenase model Complexes
A seiries of iron(III) complexes as funtionl model compounds for catechol 1,2-dioxygenases (intradiol cleavage) were synthesized and characterized by X-ray single crystal diffraction, UV-Vis spectroscopy, element analyzer, Gas chromatography –Mass and electronchemical analysises.
Enzyme, enzyme-substrate complexes and ligands:
(1) [Fe(HL1)Cl3]2
(2) [Fe(HL2)Cl3]．CH3CN
(3) [Fe(HL3)Cl3]．CH3CN
(4) [Fe(HL5)Cl3]2．CH3CN
(5) [Fe(HL6)Cl3]
(6) [Fe(HL4)(H2O)Cl2]Cl
(7) [Fe(HL5)(H2O)Cl2]Cl
(8) [Fe(L1)(C6Br4O2)]
(9) [Fe(L2)(C6Cl4O2)]
(10) [Fe(L6)(C6Cl4O2)]
(11) [Fe(HL6)(C6Br4O2)Cl]．CH3CN
(12) [Fe(HL6)2]Cl3．2 CH3CN．H2O
Catechol 1,2-dioxygenase reactivity had been performed by electronic spectrum in methanol/acetonitrile solutions. The solutions were contained Complex(1)~Complex(7) with catecholate anions of 3,5-di-tert-butylcatechol(3,5-DTBC). The results show that the high lewis acidity iron(III) complexes exhibits the higher catechol 1,2-dioxygenase reactivity. The product distribution of Catechol 1,2-dioxygenase reaction was found to be very solvent dependent.
Part Ⅱ: Iron(Ⅲ) Catecholase Reactivity Complexes
A seiries iron(III) complexes of the monophenolate ligands for catecholase activity were synthesized and characterized by X-ray single crystal diffraction, UV-Vis spectroscopy, element analyzer, and electronchemical analysises.
Enzyme-substrate complexes and ligands:
(1) [Fe(L1)(C6Br4O2)]
(2) [Fe(L2)(C6Cl4O2)]2
(3) [Fe(L3)(C6Br4O2)]．2CH3OH
(4) [Fe(L4)(C6Br4O2)]
(5) [Fe(L5)(C6Cl4O2)]．CH3CN
A seiries iron(III) compounds of the monophenolate ligands with catecholase activity had been performed by UV-Vis spectrum in DMF solutions. The solutions were contained [Fe(L1~5)Cl2] which reacted with catecholate anions of 3,5-di-tert-butylcatechol(3,5-DTBC). The results show that the high lewis acidity of iron(III) complexes exhibits the higher Catecholase reactivity.
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