Study of Iron-Gold Alloy nanoparticle for Biomedical Application

• Main Authors: Kou-Ting Wu, 吳國廷
• Other Authors: Ren-Jei Chung
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/268qch

碩士 === 國立臺北科技大學 === 工程科技研究所 === 100 === The aim of this study is to develop Iron-Gold Alloyed nanoparticles (NFAs), which are superparamagnetic and of great potential to be applied in the biomedical fields, including hyperthermia treatment for cancer and drug controlling release. The NFAs were prepared through a pyrolysis method and well dispersed in distilled water. The ratio of iron to gold was 2 : 1 through analyzing. The mean diameter was 3.932 nm. The saturated magnetization was 3.5 emu/g under 20 KOe magnetic field. The material was superparamagnetic at room temperature. The result of in vitro tests showed that under a dose of 500μg/mL were not cytotoxic to L929 cell and Hep-G2 cell. Methotrexate (MTX) is an anti-cancer medicine and able to target the cancer cells. MTX was further conjugated on to NFA through a series of chemical modifications, including 2-aminoethanethiol grafting, and then the amine bonded with the carboxyl group on MTX to form an amide bond. Immobilization of MTX on the NFA was confirmed using FTIR. The uptake of Hep-G2 cells was 0.789 pg/cell for NFA-MTX, which was 1.5 times of the L929 cells. The results indicated the targeting efficiency of NFA-MTX to cancer cells. After calculation, per NFA would generate a 2.03×10-16 J heat under a high frequency magnetic field (700 to 1100)KHz. After treating with 200μg/mL NFA-MTX and then being applied a (700 to 1100) KHz high frequency magnetic field treatment for 20 minutes, the residual viability of Hep-G2 cells dramatically decreased 45%. Besides, we also discovered that the releasing of MTX from NFA-MTX was dependent with the applied time of high frequency magnetic field. The generated heat was able to break the amide bonds between NFA and MTX. The release of MTX could be successfully controlled. Using NFA-MTX, a hyperthermia treatment and drug controlling release will be achieved under high frequency alternating magnetic field.