| Summary: | 碩士 === 東海大學 === 畜產與生物科技學系 === 100 === Egg production is an energy-intensive process in chickens. Adipose tissues are known to regulate energy balance in animals. Therefore, development of adipose tissues may play roles in animal proliferation and reproduction. Two selected lines of Taiwan country chickens originated from a common population with relatively high (L2 line) and low (B line) egg yields were used in a previous study that indicated the correlation between laying performance and the differentiation capacity, triglyceride accumulation, and lipolysis activity of adipocytes. This study aimed to further investigate the possible correlation between preadipocyte differentiation and egg production efficiency within L2 line chickens. The L2 chickens were divided into high (108.7±0.5) and low (71.3±0.5) egg production groups according to their egg productions at age of 40 weeks. Preadipocytes collected from abdominal adipose tissues were cultured and induced to differentiate. Oil-Red O staining, triglyceride content and glycerol-3-phosphate dehydrogenase (GPDH) activity assays were performed on day 6 after the induction of differentiation. The results of oil-Red O staining, triglyceride content and GPDH activity assays showed that no significant differences between adiopocytes from chickens with high and low egg productions. The mRNA expression of adipocyte differentiation- related genes were measured using real-time polymerase chain reaction. The results revealed that the lipoprotein lipase (LPL) mRNA level was higher in high egg production than those in low egg production group. However, no significant differences in the mRNA levels of peroxisome proliferator-activated receptorγ (PPARγ, fatty acid synthase (FAS), stearoyl CoA desaturase (SCD), sterol regulatory element binding protein1 (SREBP1), adiponectin, very low density lipoprotein receptor (VLDLR), perilipin, and vimentin were found between these two groups. Taken together, differentiation capacity of preadipocytes may not relate to egg productions in L2 line chickens. However the significance of LPL expression difference between L2 chickens with high and low egg productions required further study.
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