| Summary: | 碩士 === 東海大學 === 食品科學系 === 100 === It has been well known that fungal Coniothyrium sp. can produce a variety of bioactive substances, and some of these substances have already been developed as commercial biocontrol products against plant pathogens. Besides, some Coniothyrium sp. strains are capable to produce secondary metabolites with the efficacy of anti-proliferation against cancer cells. In our lab, an endemic endophytic Coniothyrium sp. CSM has displayed the ability to produce Brefeldin A (BFA), an anti-cancer compound. The objective of this study is to improve the yield of BFA produced by this fungal strain, and to search new bioactive compounds in its fermented cultures. The experiment results briefly describe as following: the yield of BFA of this strain reached 47 mg/L after eleven day fermenting by using stir tank; a fraction of cultural extract separated by using HPLC has showed anti-proliferative effect against A549 cancer cells. The best anti-proliferative performance of this fraction was to incubate cancer cells for two day at the concentration of 15μg/ml; the fraction contains several unknown compounds, according to the analysis of UPLC. These unknown compounds are worthwhile to identify in the future.
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