| Summary: | 碩士 === 東海大學 === 化學工程與材料工程學系 === 100 === The main purpose of the study is to investigate the influence of the addition of Chinese herb on the formation of bioactive components in the submerged culture of Ganoderma lucidum. In addition, the effect of types of Chinese herb and optimal addition amount were also determined.
The results show that the addition of Fagara and Turmeric could give play to the great efficiency in this way, and biomass of Fagara reaches to 8.19 g/L at the 15th day, which is 1.20 times of the contro. The polysaccharide production of Fagara reaches to 3.2 g/L at the 6th day, and is 1.60 times more than the control. The content of ganoderic acid from Turmeric addition reaches to 14.33 (mg/g D.W.) at the 15th day, and is 2.67 times of the control. In addition, the total content of polyphenol reaches to 56.32 (mg/g D.W.) at the 6th day, which is 2.74 times of the control. The content of flavonoid reaches to 4.59 (mg/g D.W.) at the 12th day, and is 3.08 times of control group. The bioactive components in Fagara and Turmeric was proved to be much better than the other Chinese herbs. In contrast, adding cinnamon extract in the beginning of the culture could inhibit the formation of mycelia. Therfore, the addition timing of cinnamon extract was also investigated, which could effectively enhance the formation of bioactive components. The results of this study are expected to be useful for the enhancement of bioactive components production in the submerged culture of Ganoderma lucidum.
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