A novel Raman fingerprint technology for rapid analysis of infectious biological materials

• Main Authors: Chang, Chia-Wen, 張嘉紋
• Other Authors: Lin, Chi-Chang
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/67482118201350811263

碩士 === 東海大學 === 化學工程與材料工程學系 === 100 === There are various microorganisms distributed widely in our nature environment. However, infectious diseases may possible increase while our immuno-system declined. Due to overuse of antibiotics, some bacteria presents their drug-resistant ability and causes the difficulty to clinical treatment. Bacteria culture is a gold standard procedure in clinical diagnostic, however, it also a complex and time consuming procedure. This research focus on a rapid screening tool, Raman spectroscopy which supports characteristics information of infectious bacteria with fast and needs only little amount samples, can be used to assist doctors in the clinical process. In the study, three bacteria including Salmonella, Acinetobacter baumannii, Klebsiella pneumonia and their related phages, and other ten Acinetobacter baumannii strains were also introduced to analysis of their Raman fingerprint. Otherwise, a novel surface enhancing Raman spectroscopy (SERS) substrate for enhancing finger print signals was also studied as part of this work. As results showed, the contains of carbohydrate in Salmonella is much higher than that of Acinetobacter baumannii and Klebsiella pneumonia. As represented by barcode analysis, these bacteria can be further identified via their characteristic peaks. Raman fingerprints of ten Acinetobacter baumannii strains showed significant peak stand at 1048 cm-1 was much higher intensity than other peaks in the group of higher-antibiotics resistant. By using gold nanoparticals with different particle size (0.9±0.3, 0.8±0.3 and 0.7±0.3 m) and the gold contains (0%, 2%, 8%, 13, 30%) as SERS substrates, the best signal enhancement condition can be founded when 0.7±0.3 m of particle size with 30% of gold contains was used.