Investigation of Microarray Data Using Gene Set Enrichment Analysis - Arabidopsis thaliana infected with Xanthomonas campestris pv. campestris

碩士 === 亞洲大學 === 生物與醫學資訊學系碩士在職專班 === 100 === Gaining a better understanding of the biotic and abiotic stress responses for plant systems provide a model system for studying human diseases and drug-related research. Understanding how plant systems defense against environment stress is of great signifi...

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Bibliographic Details
Main Authors: Ching-yi Chen, 陳靜怡
Other Authors: Ka-Lok Ng
Format: Others
Language:zh-TW
Published: 2012
Online Access:http://ndltd.ncl.edu.tw/handle/73355292561541750960
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Summary:碩士 === 亞洲大學 === 生物與醫學資訊學系碩士在職專班 === 100 === Gaining a better understanding of the biotic and abiotic stress responses for plant systems provide a model system for studying human diseases and drug-related research. Understanding how plant systems defense against environment stress is of great significance for the world's food and agricultural production.In this study, the microarray data for Arabidopsis thaliana infected with Xanthomonas campestris pv. campestris (Xcc) is analyzed. Microarray data for Arabidopsis infected with Xcc are retrieved from the ArrayExpress database, where differentially expressed genes (DEGs) and Gene Set Enrichment Analysis (GSEA) for pathogen-resistant pathways are deduced by adopting the R language, Bioconductor and KEGG information. Finally, the found results are validated by published literature.It is found that the SGT1 and HSP90 protein complexes utilize the SKp1 protein in the ubiquitin - proteasome system to regulate the hypersensitivity resistance mechanism, which is mediated by the resistance protein RPM1.Furthermore, DEG results for Xcc under different experimental conditions, as well as bacteria Agrobacterium tumefaciens, are also determined. The mentioned results can be accessed at http://ppi.bioinfo.asia.edu.tw/R_At_xcc/index.htm .