| Summary: | 碩士 === 慈濟大學 === 分子生物暨人類遺傳學系碩士班 === 100 === During carcinogenesis, cellular growth is altered by over-signals and insensitivity to the antigrowth signals and evasion of apoptosis. Finally, cells will limitless replication and metastasis. Regulation of the cell cycle by a chemical compound would be a new therapy strategy. The aim of our study was to examine the antiproliferation activities of plant extracts (eight plants, Ligustrum lucidum (LL), Astragalus membranaceus (AM), cuscuta chinensis (CC), Alternanthera philoxeroides (AP), Ludwigia octovalvis (LO), Plantago asiatica (PA), Morus australis poir (MAP) and Kalanchoe tubiflora (KT)). The different plant extracts to affect cell viability and cell cycle was evaluated by HeLa cell. Six ethanol extracts and two water extracts decrease cell survival ration to 60% below 400 μg/ml. The most interesting things were that LL and KT-ethanol extracts inhibit cell proliferation and affect cell morphology. KT-EtOH also induce an accumulation of cells in the G2/M phase of the cell cycle as well as an increased level of cells in the subG1 phase. KT-EtOH inhibits cell survivor by two mechanisms that are exclusively in dividing cells: first, disrupting centrosome integrity and inducing multipolarity; second, disrupting chromosome alignment at metaphase. The EtOH extract of LL was able to inhibit cell proliferation. After 48 hr treatment with 0.4 mg/ml of LL, the cell viability was less than 50% of controls, and changed cell morphology, LL affected nucleus envelope and multinucleus.
|
|---|
