The role of antioxidants in human ADSCs culture condition

• Main Authors: Yi-Yu Chou, 周怡佑
• Other Authors: Ching-Feng Cheng
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/93337215596383506648

碩士 === 慈濟大學 === 生理暨解剖醫學碩士班 === 100 === Human mesenchymal stem cells (hMSCs) have been isolated from bone marrow and other adult tissues and are potentially useful for tissue engineering. Adipose tissue has several clear advantages as a starting material for harvesting stem cells, as it is abundant and relatively easy to procure. However, existing methods to expand adipose-derived MSCs (ADSC) are not optimal condition. In this study, we described a protocol using a modified cell culture medium that accelerates greatly the growth rate of ADSCs. Our result was accomplished by using a growth medium supplemented with N-acetyl-L-cysteine and L-ascorbic acid-2-phosphate.Measuring by total cell number, the proliferation of ADSCs was significantly higher than prior reports listed in the literature. In addition, cells produced early in these culture medium displayed similar characteristics to those previously reported for multipotential stem cells, including the fibroblast-like morphology and could readily be induced to differentiate into adipocytes and osteoblasts. Therefore, addition of antioxidants into growth medium greatly enhanced the growth rate of adipose-derived multipotential human MSCs without losing its stem cell characteristics.