The Role of Map4k3 Gene in Adipogenesis

• Main Authors: Yun-ya Huang, 黃筠雅
• Other Authors: Jih-i Yeh
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/29994797850137184041

碩士 === 慈濟大學 === 生命科學系碩士班 === 100 === Obesity is a state of excessive white adipose tissue. The glucose uptake rate of fat cells increases in response to insulin stimulation. Despite larger adipose tissue mass, obese people respond less to insulin stimulation. Our laboratory studies adipogenesis in cell culture models. Screening of mouse kinase shRNA library showed that MAP4K3 (mitogen-activated protein kinase kinase kinase kinase 3) knockdown enhanced adipogenesis. 3T3-L1 cells can be morphologically transformed into adipocytes without 3-isobutyl-1-methylxanthine (MIX) in the differentiation medium after MAP4K3 knockdown. Both basal and insulin stimulated glucose uptake rate increased after MAP4K3 knockdown in 3T3-L1 adipocytes and qPCR showed expression of CBP300 and PCAF decreased. These results showed the important roles of MAP4K3 in adipogenesis and insulin response. Further studies will help elucidate the mechanism of MAP4K3 inhibition of insulin sensitivity. studies