| Summary: | 碩士 === 大仁科技大學 === 生物科技研究所 === 100 === The blue shark (Prionace glauca) is a species of requiem shark, family Carcharhinidae, that has cartilage axial skeleton. The main constituents of cartilage are collagen, glycosaminoglycan and a variety of proteins. The scientific literatures showed that the active substances of shark cartilage could inhibit angiogenesis, tumor formation and inflammation. The objective of this study was to investigate the anti - inflammation and immune modulatory effects of blue shark cartilage alkaline extract (BSC-AE) and purify extract (BSC-PE) by in vitro using primary cells from BALB/c mice. The BSC-AE was extracted from shark cartilage by alkali extraction, following ethanol precipitation to obtain BSC-PE, and chondroitin sulfate content of extracts was analyzed by high-performance liquid chromatography (HPLC). The cell viability was determined by MTT (3- (4,5-cimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) test and cytokines secreted in the spleen and peritoneal cell cultures were quantified by ELISA (Enzyme-Linked Immunosorbent Assay). The results showed that chondroitin sulfate contents of BSC-PE and BSC-AE were 48.40% and 10.16% respectively. In spontaneous pattern, BSC-AE and BSC-PE significantly (P <0.05) increased the interleukin (IL)-10 level that secreted by splenocytes. The high dose (1000 μg/ml) of BSC-AE was significantly (P <0.05) decreased pro-inflammatory cytokine (IL-6) level and pro-inflammatory and anti-inflammatory cytokine (IL-6/IL-10) ratio in both of lipopolysaccharide (LPS)- and concanavalin A (Con A)- stimulated splenocyte cultures, it also significantly (P <0.05) reduced the IL-6 levels in the spontaneous and LPS-stimulated peritoneal cell cultures. The BSC-AE (125-1000 μg/ml) markedly (P <0.05) inhibited the secretion of helper T cell type II (Th2) cytokine (IL-4), increased the ratios of Th1/Th2 cytokines, interferon-γ (IFN-γ)/IL-4, in the Con A-stimulated splenocyte cultures. Besides, the IFN-γ levels were significantly (P <0.05) improved in the splenocyte cultures that stimulated by BSC-PE and BSC-AE. The results suggest that the blue shark cartilage extracts, BSC-AE and BSC-PE, might have the potential to alleviate the inflammation and hypersensitivity in allergic diseases (such as asthma) via modulating the Th1/Th2 balance toward Th1 pole.
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